Effectiveness of Phosphorus and Nitrogen Removal in the Water Treatment Process at Khmer Beverages, Phnom Penh
Abstract
Purpose of the study: The aim of this study is to understand the process of water treatment in each step and to understand the effectiveness of the removal of phosphorus and nitrogen from wastewater.
Methodology: For nitrogen removal must be through many processes such as Nitrogen fixation, or Ammonification, and Denitrification. For the phosphorus. The additional Ferric Chloride in the Aeration tanks to a reaction between the melt and the mud bolts is separated at the Clarifier tank. The Coagulation basin adds Ferric Chloride at 40% concentration of 0.05ml in contaminated water 1000ml for nitrogen and phosphorus concentration tests and studied in the condition the nitrogen can be removed from contaminated water well depending on the pH value and temperature. The removal of phosphorus by adding 0.05ml ferric chloride and 5ml of polymer cation 1040 (powder) can tank up to 80% phosphorus at the temperature of 200C and pH 7.
Main Findings: As a result, the good conditions for nitrification are the pH of between 7.5 and 8.6 at temperature in the tank between 200C and 250C and the denitrification has a pH of between 7.5 and 8 and temperature in the tank from 200C to 350C. Result show that the remaining phosphorus is below the ministry of environment standard set (P <2mg/l).
Novelty/Originality of this study: The next study should observe the amount of polymer to be applied after adding iron and studying the speed of iron chips as it affects the removal of phosphorus.
References
United Nations Industrial Development Organization (UNIDO), Industrial Development Report 2020: Industrializing in the Digital Age, 2020.
World Health Organization (WHO), Guidelines for Drinking-Water Quality, 4th ed., Geneva, Switzerland: WHO, 2017.
O. S. Amuda, I. A. Amoo, and O. O. Ajayi, “Performance optimization of coagulant/flocculant in the treatment of wastewater from a beverage industry,” J. Hazard. Mater., vol. 129, no. 1–3, pp. 69–72, Feb. 2006.
S. K. Ramasahayam, L. Guzman, G. Gunawan, and T. Viswanathan, “A comprehensive review of phosphorus removal technologies and processes,” J. Macromol. Sci. A, vol. 51, no. 6, pp. 538–545, 2014.
J. Thistleton, J. Smith, and A. Hurst, “Mechanisms of chemical phosphorus removal: 1—Iron (II), Iron (III) and Aluminium (III) Salts,” Water Environ. J., vol. 15, no. 2, pp. 112–117, Jun. 2001.
I. R. de Barros et al., “Kinetics of the precipitation reaction between aluminium and orthophosphate species,” J. Water Process Eng., vol. 57, p. 104689, Feb. 2024.
Z. Qin et al., “Mechanisms of phosphorus removal by ferrate (VI),” Water Res., vol. 139, pp. 139–146, Jul. 2018.
F. Wang et al., “Phosphate recovery from swine wastewater by a struvite-based precipitation process,” Sci. Rep., vol. 9, no. 1, p. 9131, Jun. 2019.
G. Radu et al., “Kinetics and chemistry of nitrification process – a review,” Environ. Eng. Manag. J., vol. 20, no. 1, pp. 15–22, Jan. 2021.
A. R. Dinçer and F. Kargı, “Kinetics of sequential nitrification and denitrification processes,” Enzyme Microb. Technol., vol. 27, no. 1–2, pp. 37–42, Jul. 2000.
S. Oktay, G. Iskender, F. G. Babuna, G. Kutluay, and D. Orhon, “Improving the wastewater management for a beverage industry with in-plant control,” Desalination, vol. 208, no. 1–3, pp. 1–10, Apr. 2007.
E. Ait Hsine, A. Benhammou, and M. N. Pons, “Design of a beverage industry wastewater treatment facility using process simulation,” IFAC Proc. Vol., vol. 37, no. 2, pp. 249–254, 2004.
A. Bhambri et al., “In-situ remediation of nitrogen and phosphorus of beverage industry by potential strains Bacillus sp. (BK1) and Aspergillus sp. (BK2),” Sci. Rep., vol. 11, no. 1, p. 12049, Jun. 2021.
N. Wei et al., “Coagulation behavior of polyaluminum chloride: Effects of pH and coagulant dosage,” Chin. J. Chem. Eng., vol. 23, no. 5, pp. 893–899, May 2015.
P. D. Johnson et al., “Enhanced removal of heavy metals in primary treatment using ferric chloride,” Water Environ. Res., vol. 89, no. 7, pp. 617–626, Jul. 2017.
C. S. Lee, J. Robinson, and M. F. Chong, “A review on application of flocculants in wastewater treatment,” Process Saf. Environ. Prot., vol. 92, no. 6, pp. 489–508, Nov. 2014.
X. Liu et al., “Understanding the coagulation mechanism and floc properties induced by Fe(VI) and FeCl3: population balance modeling,” Water Sci. Technol., vol. 83, no. 10, pp. 2377–2390, May 2021.
L. S. Kang and J. L. Cleasby, “Temperature effects on flocculation kinetics using Fe (III) coagulant,” J. Environ. Eng., vol. 121, no. 12, pp. 893–901, Dec. 1995.
D. Guan, Z. Zhang, and X. Li, “Effect of pH and temperature on coagulation efficiency in a North-China water treatment plant,” in Advanced Materials Research, vol. 243, pp. 4835–4839, 2011.
X. Lu et al., “Research on intelligent chemical dosing system for phosphorus removal in wastewater treatment plants,” Water, vol. 16, no. 11, p. 1623, Jun. 2024.
Veolia, “Discharge requirements for wastewater,” Internal Document, 2007.
G. Tchobanoglous, F. L. Burton, and H. D. Stensel, Wastewater Engineering: Treatment and Reuse, 4th ed. New York, NY, USA: McGraw-Hill, 2003.
B. Shi et al., “The effect of pH on the precipitation of phosphate from wastewater using ferric chloride,” Water Res., vol. 36, no. 1, pp. 14–20, Jan. 2002.
M. Henze, M. C. M. van Loosdrecht, G. A. Ekama, and D. Brdjanovic, Biological Wastewater Treatment: Principles, Modelling and Design. London, UK: IWA Publishing, 2008.
A. H. El-Shazly et al., “Kinetics and performance of phosphate removal from hot spring water using ferric chloride,” J. Environ. Manage., vol. 128, pp. 800–807, Oct. 2013.
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