Schrödinger: Journal of Physics Education
Schrödinger: Journal of Physics Education

Advancing Physics and Physics Education Through Research and Innovation

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Schrödinger: Journal of Physics Education

Advancing Physics and Physics Education Through Research and Innovation

Combined Ultrasonic and Thermal Treatment: Effects on Microbial Reduction and Protein Stability in Fresh Milk

Mei Chi Lee
Universiti of Malaysia Sabah ROR
Malaysia
Atul Handayani
Maulana Malik Ibrahim State Islamic University
Indonesia
atulhandayani11@gmail.com
Sing Huat Po
University of Brunei Darussalam ROR
Brunei Darussalam
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  • Purpose of the study: This study aims to evaluate the effect of ultrasonic treatment combined with temperature variation on the inhibition of Escherichia coli and the preservation of protein content in fresh cow’s milk under controlled experimental conditions.

    Methodology: Experimental design; ultrasonic generator (60 kHz, 70 W); water bath temperature control (30°C, 40°C, 50°C); incubator shaker; laminar air flow; Total Plate Count (TPC) method; Kjeldahl method; colony counter; Nutrient Agar (NA) and Nutrient Broth (NB); two-way ANOVA; descriptive statistics; statistical software (SPSS).

    Main Findings: Ultrasonic treatment combined with temperature significantly reduced Escherichia coli counts, with the highest reduction (95.9%) achieved at 50°C for 30 minutes. Bacterial counts decreased progressively with increasing temperature and exposure time. Protein content remained relatively stable, ranging from 2.02% to 2.20%, indicating minimal degradation under treatment conditions.

    Novelty/Originality of this study: This study presents an integrated and statistically validated approach to simultaneously analyze microbial inactivation and protein stability using combined ultrasonic and thermal treatments. It demonstrates a synergistic interaction between acoustic cavitation and moderate temperature, offering a novel framework linking physical wave principles with biological systems in food processing.

  • How to cite

    [1]
    M. C. Lee, A. Handayani, and S. H. Po, “Combined Ultrasonic and Thermal Treatment: Effects on Microbial Reduction and Protein Stability in Fresh Milk”, Sch. Jo. Phs. Ed, vol. 7, no. 2, pp. 95–104, Apr. 2026, doi: 10.37251/sjpe.v7i2.3082.

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    1. C. Lauteri, G. Ferri, A. Piccinini, L. Pennisi, and A. Vergara, “Ultrasound technology as inactivation method for foodborne pathogens: A review,” 2023. doi: 10.3390/foods12061212. DOI: https://doi.org/10.3390/foods12061212
    2. D. Grace, “Burden of foodborne disease in low-income and middle-income countries and opportunities for scaling food safety interventions,” Food Secur., vol. 15, no. 6, pp. 1475–1488, 2023, doi: 10.1007/s12571-023-01391-3. DOI: https://doi.org/10.1007/s12571-023-01391-3
    3. C. C. Adley and M. P. Ryan, “Chapter 1 - The Nature and Extent of Foodborne Disease,” J. B. T.-A. F. P. (Second E. Barros-Velázquez, Ed., San Diego: Academic Press, 2025, pp. 3–14. doi: 10.1016/B978-0-323-90747-7.00002-8. DOI: https://doi.org/10.1016/B978-0-323-90747-7.00002-8
    4. K. Rathnakumar et al., “Ultrasonic processing: effects on the physicochemical and microbiological aspects of dairy products,” Crit. Rev. Biotechnol., vol. 44, no. 8, pp. 1638–1652, Nov. 2024, doi: 10.1080/07388551.2024.2332941. DOI: https://doi.org/10.1080/07388551.2024.2332941
    5. A. S. El-Sayed, H. Ibrahim, and M. A. Farag, “Detection of potential microbial contaminants and their toxins in fermented dairy products: A comprehensive review,” Food Anal. Methods, vol. 15, no. 7, pp. 1880–1898, 2022, doi: 10.1007/s12161-022-02253-y. DOI: https://doi.org/10.1007/s12161-022-02253-y
    6. E. A. Alexa, A. Papadochristopoulos, T. O’Brien, and C. M. Burgess, “Chapter 1 - Microbial contamination of food,” A. K. Jaiswal and S. B. T.-F. P. and P. Shankar, Eds., Academic Press, 2024, pp. 3–19. doi: 10.1016/B978-0-323-90044-7.00001-X. DOI: https://doi.org/10.1016/B978-0-323-90044-7.00001-X
    7. S. Karanth, S. Feng, D. Patra, and A. K. Pradhan, “Linking microbial contamination to food spoilage and food waste: the role of smart packaging, spoilage risk assessments, and date labeling,” Front. Microbiol., vol. Volume 14-2023, 2023, doi: 10.3389/fmicb.2023.1198124 DOI: https://doi.org/10.3389/fmicb.2023.1198124
    8. O. Misiou and K. Koutsoumanis, “Climate change and its implications for food safety and spoilage,” Trends Food Sci. Technol., vol. 126, pp. 142–152, 2022, doi: 10.1016/j.tifs.2021.03.031. DOI: https://doi.org/10.1016/j.tifs.2021.03.031
    9. G. K. Mahunu, M. Osei-Kwarteng, M. C. Ogwu, and N. A. Afoakwah, “Safe Food Handling Techniques to Prevent Microbial Contamination BT - Food Safety and Quality in the Global South,” M. C. Ogwu, S. C. Izah, and N. R. Ntuli, Eds., Singapore: Springer Nature Singapore, 2024, pp. 427–461. doi: 10.1007/978-981-97-2428-4_14. DOI: https://doi.org/10.1007/978-981-97-2428-4_14
    10. B. Mengstu et al., “Evaluation of microbial hygiene indicators in raw milk, pasteurised milk and cottage cheese collected across the dairy value chain in Ethiopia,” Int. Dairy J., vol. 136, p. 105487, 2023, doi: 10.1016/j.idairyj.2022.105487. DOI: https://doi.org/10.1016/j.idairyj.2022.105487
    11. A. Deddefo, G. Mamo, M. Asfaw, and K. Amenu, “Factors affecting the microbiological quality and contamination of farm bulk milk by Staphylococcus aureus in dairy farms in Asella, Ethiopia,” BMC Microbiol., vol. 23, no. 1, p. 65, 2023, doi: 10.1186/s12866-022-02746-0. DOI: https://doi.org/10.1186/s12866-022-02746-0
    12. D. T. Robi, T. Mossie, and S. Temteme, “A comprehensive review of the common bacterial infections in dairy calves and advanced strategies for health management,” Vet. Med. Res. Reports, vol. 15, no. null, pp. 1–14, Dec. 2024, doi: 10.2147/VMRR.S452925. DOI: https://doi.org/10.2147/VMRR.S452925
    13. M. A. Alhadlaq et al., “Overview of pathogenic Escherichia coli, with a focus on Shiga toxin-producing serotypes, global outbreaks (1982–2024) and food safety criteria,” Gut Pathog., vol. 16, no. 1, p. 57, 2024, doi: 10.1186/s13099-024-00641-9. DOI: https://doi.org/10.1186/s13099-024-00641-9
    14. A. A. Akinsemolu and H. N. Onyeaka, “Microorganisms Associated with Food Spoilage and Foodborne Diseases BT - Food Safety and Quality in the Global South,” M. C. Ogwu, S. C. Izah, and N. R. Ntuli, Eds., Singapore: Springer Nature Singapore, 2024, pp. 489–531. doi: 10.1007/978-981-97-2428-4_16. DOI: https://doi.org/10.1007/978-981-97-2428-4_16
    15. M. Mkangara, “Prevention and control of human salmonella enterica infections: An implication in food safety,” Int. J. Food Sci., vol. 2023, no. 1, p. 8899596, Jan. 2023, doi: 10.1155/2023/8899596. DOI: https://doi.org/10.1155/2023/8899596
    16. A. Kaushik, N. K. Taneja, V. K. Juneja, J. K. Salazar, A. Joshi, and H. S. Oberoi, “Enhancing microbial safety and quality of milk with ultrasonication: Kinetics modeling of pathogenic Bacteria and milk characteristics,” LWT, vol. 202, p. 116287, 2024, doi: 10.1016/j.lwt.2024.116287. DOI: https://doi.org/10.1016/j.lwt.2024.116287
    17. E. J. Sarba, W. Wirtu, E. Z. Gebremedhin, B. M. Borena, and L. M. Marami, “Occurrence and antimicrobial susceptibility patterns of Escherichia coli and Escherichia coli O157 isolated from cow milk and milk products, Ethiopia,” Sci. Rep., vol. 13, no. 1, p. 16018, 2023, doi: 10.1038/s41598-023-43043-8. DOI: https://doi.org/10.1038/s41598-023-43043-8
    18. B. Gume et al., “Bacterial hazard identification and exposure assessment of raw milk consumption in Jimma zone, South West Ethiopia,” BMC Microbiol., vol. 23, no. 1, p. 166, 2023, doi: 10.1186/s12866-023-02910-0. DOI: https://doi.org/10.1186/s12866-023-02910-0
    19. D. Bermudez-Aguirre and B. A. Niemira, “Pasteurization of foods with ultrasound: The present and the future,” 2022. doi: 10.3390/app122010416. DOI: https://doi.org/10.3390/app122010416
    20. M. Azizi-Lalabadi, N. R. Moghaddam, and S. M. Jafari, “9 - Pasteurization in the food industry,” S. M. B. T.-T. P. of F. P. by S. and H. W. Jafari, Ed., Woodhead Publishing, 2023, pp. 247–273. doi: 10.1016/B978-0-12-818616-9.00009-2. DOI: https://doi.org/10.1016/B978-0-12-818616-9.00009-2
    21. K. K. Dash et al., “A comprehensive review on heat treatments and related impact on the quality and microbial safety of milk and milk-based products,” Food Chem. Adv., vol. 1, p. 100041, 2022, doi: 10.1016/j.focha.2022.100041. DOI: https://doi.org/10.1016/j.focha.2022.100041
    22. N. Nikmaram and K. M. Keener, “The effects of cold plasma technology on physical, nutritional, and sensory properties of milk and milk products,” LWT, vol. 154, p. 112729, 2022, doi: 10.1016/j.lwt.2021.112729. DOI: https://doi.org/10.1016/j.lwt.2021.112729
    23. I. Neoκleous, J. Tarapata, and P. Papademas, “Non-thermal processing technologies for dairy products: Their effect on safety and quality characteristics,” Front. Sustain. Food Syst., vol. Volume 6-, 2022, doi: 10.3389/fsufs.2022.856199. DOI: https://doi.org/10.3389/fsufs.2022.856199
    24. D. Verma et al., “Feasibility of pulse protein in the formulation of dairy alternatives: a review of nutri-functional attributes, applications, processing, and sustainability impact,” Nutrire, vol. 50, no. 1, p. 12, 2025, doi: 10.1186/s41110-025-00315-9. DOI: https://doi.org/10.1186/s41110-025-00315-9
    25. A. Karim et al., “1 - Introduction to thermal food processes by steam and hot water,” S. M. B. T.-T. P. of F. P. by S. and H. W. Jafari, Ed., Woodhead Publishing, 2023, pp. 3–26. doi: 10.1016/B978-0-12-818616-9.00001-8. DOI: https://doi.org/10.1016/B978-0-12-818616-9.00001-8
    26. Ž. Pandur, J. Zevnik, D. Podbevšek, B. Stojković, D. Stopar, and M. Dular, “Water treatment by cavitation: Understanding it at a single bubble - bacterial cell level,” Water Res., vol. 236, p. 119956, 2023, doi: 10.1016/j.watres.2023.119956. DOI: https://doi.org/10.1016/j.watres.2023.119956
    27. A. T. Mustapha, H. Wahia, Q. Ji, O. A. Fakayode, L. Zhang, and C. Zhou, “Multiple-frequency ultrasound for the inactivation of microorganisms on food: A review,” J. Food Process Eng., vol. 47, no. 4, p. e14587, Apr. 2024, doi: 10.1111/jfpe.14587. DOI: https://doi.org/10.1111/jfpe.14587
    28. M. Siddique, R. Rashid, and A. Ali, “Chapter 1 - Fundamentals of acoustic cavitation, ultrasound-assisted processes, and sonochemistry,” K. B. T.-M. and S. of S.-P. Kerboua, Ed., Elsevier, 2025, pp. 3–17. doi: 10.1016/B978-0-443-23651-8.00001-2. DOI: https://doi.org/10.1016/B978-0-443-23651-8.00001-2
    29. A. Gholamhosseinpour, S. M. B. Hashemi, and H. Khosravi Mazydi, “Ultrasound-assisted enhancement of microbial, physicochemical, and bioactive properties in Lactobacillus helveticus-fermented milk,” Food Chem. X, vol. 29, p. 102724, 2025, doi: 10.1016/j.fochx.2025.102724. DOI: https://doi.org/10.1016/j.fochx.2025.102724
    30. Y. Alves de Aguiar Bernardo, D. Kaic Alves do Rosario, and C. Adam Conte-Junior, “Ultrasound on milk decontamination: Potential and limitations against foodborne pathogens and spoilage bacteria,” Food Rev. Int., vol. 39, no. 1, pp. 320–333, Jan. 2023, doi: 10.1080/87559129.2021.1906696. DOI: https://doi.org/10.1080/87559129.2021.1906696
    31. S. Condón-Abanto, C. Arroyo, I. Álvarez, S. Condón, and J. G. Lyng, “Application of ultrasound in combination with heat and pressure for the inactivation of spore forming bacteria isolated from edible crab (Cancer pagurus).,” Int. J. Food Microbiol., vol. 223, pp. 9–16, Apr. 2016, doi: 10.1016/j.ijfoodmicro.2016.02.001. DOI: https://doi.org/10.1016/j.ijfoodmicro.2016.02.001
    32. M. Singla and N. Sit, “Application of ultrasound in combination with other technologies in food processing: A review.,” Ultrason. Sonochem., vol. 73, p. 105506, May 2021, doi: 10.1016/j.ultsonch.2021.105506. DOI: https://doi.org/10.1016/j.ultsonch.2021.105506
    33. B. Khadhraoui, V. Ummat, B. K. Tiwari, A. S. Fabiano-Tixier, and F. Chemat, “Review of ultrasound combinations with hybrid and innovative techniques for extraction and processing of food and natural products,” Ultrason. Sonochem., vol. 76, p. 105625, 2021, doi: 10.1016/j.ultsonch.2021.105625. DOI: https://doi.org/10.1016/j.ultsonch.2021.105625
    34. X. Chen et al., “A comparative study of ultrasound and thermal processing: Effects on goat milk stability and protein structure,” J. Dairy Sci., vol. 108, no. 4, pp. 3273–3286, 2025, doi: 10.3168/jds.2024-25918. DOI: https://doi.org/10.3168/jds.2024-25918
    35. A. T. Hong Bui, D. Cozzolino, B. Zisu, and J. Chandrapala, “Infrared analysis of ultrasound treated milk systems with different levels of caseins, whey proteins and fat,” Int. Dairy J., vol. 117, p. 104983, 2021, doi: 10.1016/j.idairyj.2021.104983. DOI: https://doi.org/10.1016/j.idairyj.2021.104983
    36. I. Lambropoulos and I. G. Roussis, “Inhibition of the decrease of volatile esters and terpenes during storage of a white wine and a model wine medium by caffeic acid and gallic acid,” Food Res. Int., vol. 40, no. 1, pp. 176–181, 2007, doi: 10.1016/j.foodres.2006.09.003. DOI: https://doi.org/10.1016/j.foodres.2006.09.003
    37. S. H. K., P. Adhikari, A. E. Yasmeen Shaikh, and S. S. Arya, “Hydrodynamic and ultrasonic cavitation physically modifies the milk protein concentrates with improved functionality,” Sustain. Food Technol., vol. 4, no. 1, pp. 1004–1020, 2026, doi: 10.1039/D5FB00393H. DOI: https://doi.org/10.1039/D5FB00393H
    38. Y. Sun et al., “Influence of milk fat globule membrane and milk protein concentrate treated by ultrasound on the structural and emulsifying stability of mimicking human fat emulsions.,” Ultrason. Sonochem., vol. 82, p. 105881, Jan. 2022, doi: 10.1016/j.ultsonch.2021.105881. DOI: https://doi.org/10.1016/j.ultsonch.2021.105881
    39. A. Prosekov, “The effect of ultrasound on the functionality and health-improving properties of dairy products (review),” Food Prod. Process. Nutr., vol. 7, no. 1, p. 36, 2025, doi: 10.1186/s43014-025-00311-6. DOI: https://doi.org/10.1186/s43014-025-00311-6
    40. O. Krasulya, S. Shestakov, V. Bogush, I. Potoroko, P. Cherepanov, and B. Krasulya, “Applications of sonochemistry in Russian food processing industry,” Ultrason. Sonochem., vol. 21, no. 6, pp. 2112–2116, 2014, doi: 10.1016/j.ultsonch.2014.03.015. DOI: https://doi.org/10.1016/j.ultsonch.2014.03.015
    41. S. Manickam et al., “Ultrasonics and sonochemistry: Editors’ perspective,” Ultrason. Sonochem., vol. 99, p. 106540, 2023, doi: 10.1016/j.ultsonch.2023.106540. DOI: https://doi.org/10.1016/j.ultsonch.2023.106540
    42. D. Fernandez Rivas, P. Cintas, J. Glassey, and D. C. Boffito, “Ultrasound and sonochemistry enhance education outcomes: From fundamentals and applied research to entrepreneurial potential.,” Ultrason. Sonochem., vol. 103, p. 106795, Feb. 2024, doi: 10.1016/j.ultsonch.2024.106795. DOI: https://doi.org/10.1016/j.ultsonch.2024.106795