Sustainable Nutrient Management for NFT Hydroponic Lettuce: Integrating Kipahit (Tithonia diversifolia) Liquid Organic Fertilizer with AB-Mix
Abstract
Purpose of the study: This study aims to determine the optimal combination of kipahit liquid organic fertilizer concentration and AB Mix to enhance the growth and production of green lettuce using the nutrient film technique in hydroponics.
Methodology: This study used a completely randomized design with 5 treatments, namely P1 = liquid organic fertilizer kipahit 0% + AB Mix 100% (control), P2 = liquid organic fertilizer kipahit 25%+AB Mix 75%, P3 = liquid organic fertilizer kipahit 50%+AB Mix 50%, P4= liquid organic fertilizer kipahit 75%+AB Mix 25%, P5=POC kipahit 100% + AB Mix 0%. Data analysis used the test of variance (ANOVA) and the DMRT follow-up test at the 5% level.
Main Findings: The provision of AB Mix and liquid organic fertilizer Kipahit nutrition had a significant effect on all parameters. P2 is a combination treatment of AB Mix and liquid organic fertilizer Kipahit, which can reduce the use of AB Mix nutrients on the parameters of plant height, leaf length, leaf width, number of leaves, and plant wet weight, but cannot increase plant growth and production of green lettuce compared to the use of 100% AB Mix. No combination of kipahit liquid organic fertilizer concentration and Ab Mix could increase the growth and production of green lettuce compared to using 100% AB Mix.
Novelty/Originality of this study: This study provides new insights into the potential of kipahit liquid organic fertilizer as a partial substitute for AB Mix in NFT hydroponics for lettuce production.
References
R. Islam et al., “Evaluation of lettuce growth, yield, and economic viability grown vertically on unutilized building wall in Dhaka City,” Front. Sustain. Cities, vol. 3, no. March, pp. 1–10, 2021, doi: 10.3389/frsc.2021.582431.
M. M. Khan, M. T. Akram, R. Janke, R. W. K. Qadri, A. M. Al-Sadi, and A. A. Farooque, “Urban horticulture for food secure cities through and beyond covid-19,” Sustain., vol. 12, no. 22, pp. 1–21, 2020, doi: 10.3390/su12229592.
S. M. E. Rahman, M. A. Mele, Y. T. Lee, and M. Z. Islam, “Consumer preference, quality, and safety of organic and conventional fresh fruits, vegetables, and cereals,” Foods, vol. 10, no. 1, pp. 1–17, 2021, doi: 10.3390/foods10010105.
L. C. Huang, “Consumer attitude, concerns, and brand acceptance for the vegetables cultivated with sustainable plant factory production systems,” Sustain., vol. 11, no. 18, 2019, doi: 10.3390/su11184862.
R. S. Velazquez-Gonzalez, A. L. Garcia-Garcia, E. Ventura-Zapata, J. D. O. Barceinas-Sanchez, and J. C. Sosa-Savedra, “A review on hydroponics and the technologies associated for medium-and small-scale operations,” Agric., vol. 12, no. 5, pp. 1–21, 2022, doi: 10.3390/agriculture12050646.
H. R. Atherton and P. Li, “Hydroponic cultivation of medicinal plants—plant organs and hydroponic systems: techniques and trends,” Horticulturae, vol. 9, no. 3, pp. 1–26, 2023, doi: 10.3390/horticulturae9030349.
S. Shilpa, P. Sharma, and Bansuli, “Hydroponics in vegetable crops: a review BT - hdroponics: the future of sustainable farming,” N. Kumar, Ed., New York, NY: Springer US, 2024, pp. 15–41. doi: 10.1007/978-1-0716-3993-1_2.
M. E. A. Elsallam, S. H. EL-Moslamy, A. A. El-Al, and H. F. Zahran, “Scaling-up production of cost-effective and eco-friendly bio-fertilizer and its application on Barley green fodder via IoT hydroponic system,” J. Genet. Eng. Biotechnol., vol. 19, no. 1, pp. 1–12, 2021, doi: 10.1186/s43141-021-00196-1.
O. D. Palmitessa, A. Signore, and P. Santamaria, “Advancements and future perspectives in nutrient film technique hydroponic system: a comprehensive review and bibliometric analysis,” Front. Plant Sci., vol. 15, no. December, pp. 1–21, 2024, doi: 10.3389/fpls.2024.1504792.
S. A. Gillani, R. Abbasi, P. Martinez, and R. Ahmad, “Comparison of energy-use efficiency for lettuce plantation under nutrient film technique and deep-water culture hydroponic systems,” Procedia Comput. Sci., vol. 217, pp. 11–19, 2023, doi: 10.1016/j.procs.2022.12.197.
M. R. Rozilan, A. S. Mohd Rodzi, A. Faiz Zubair, A. R. Hemdi, R. Deraman, and N. D. Md Sin, “Design and fabrication of nutrient film technique (NFT) hydroponic system,” in M. H. A. Hassan, M. H. Zohari, K. Kadirgama, N. A. N. Mohamed, and A. Aziz, Eds., Technological Advancement in Instrumentation & Human Engineering. ICMER 2021. Lecture Notes in Electrical Engineering, vol 882. Springer, Singapore, 2023, pp. 123–144.
G. Carrasco et al., “Enhancing leafy greens’ production: nutrient film technique systems and automation in container-based vertical farming,” Agronomy, vol. 14, no. 9, pp. 1–19, 2024, doi: 10.3390/agronomy14091932.
A. Mielcarek, K. Kłobukowska, J. Rodziewicz, W. Janczukowicz, and K. Ł. Bryszewski, “Water nutrient management in soilless plant cultivation versus sustainability,” Sustain., vol. 16, no. 1, 2024, doi: 10.3390/su16010152.
M. Manna, B. Rengasamy, and A. K. Sinha, “Nutrient and water availability influence rice physiology, root architecture and ionomic balance via auxin signalling,” Plant. Cell Environ., vol. 48, no. 4, pp. 2691–2705, Apr. 2025, doi: 10.1111/pce.15171.
E. Fathidarehnijeh, M. Nadeem, M. Cheema, R. Thomas, M. Krishnapillai, and L. Galagedara, “Current perspective on nutrient solution management strategies to improve the nutrient and water use efficiency in hydroponic systems,” Can. J. Plant Sci., vol. 104, no. 2, pp. 88–102, 2024, doi: 10.1139/cjps-2023-0034.
O. A. Nitu, E. Ş. Ivan, A. S. Tronac, and A. Arshad, “Optimizing lettuce growth in nutrient film technique hydroponics: evaluating the impact of elevated oxygen concentrations in the root zone under LED illumination,” Agronomy, vol. 14, no. 9, 2024, doi: 10.3390/agronomy14091896.
E. Bwambale, F. K. Abagale, and G. K. Anornu, “Smart irrigation monitoring and control strategies for improving water use efficiency in precision agriculture: A review,” Agric. Water Manag., vol. 260, p. 107324, 2022, doi: 10.1016/j.agwat.2021.107324.
M. Mallareddy et al., “Maximizing water use efficiency in rice farming: A comprehensive review of innovative irrigation management technologies,” Water (Switzerland), vol. 15, no. 10, 2023, doi: 10.3390/w15101802.
N. D. S. Sanusi, F. C. Ros, S. Z. N. Demon, I. Ismail, and I. Hamadneh, “Recent use of AB mix nutrients for urban farming in Malaysia: a mini review,” J. Adv. Res. Des., vol. 122, no. 1, pp. 150–162, 2024, doi: 10.37934/ard.122.1.150162.
M. Hayati, D. M. Indriani, and M. Rahmawati, “The effect of AB mix and organic fertilizer concentration on growth and yield of two mustard varieties in a floating hydroponic system,” IOP Conf. Ser. Earth Environ. Sci., vol. 1183, no. 1, 2023, doi: 10.1088/1755-1315/1183/1/012034.
B. Hermanto, D. Habibie, A. F. Lubis, and R. A. Syahputra, “Analysis of Pakcoy Mustard (Brassica rapa) growth using hydroponic system with AB mix nutrition,” J. Phys. Conf. Ser., vol. 1819, no. 1, 2021, doi: 10.1088/1742-6596/1819/1/012059.
K. Kano et al., “Effects of organic fertilizer on bok choy growth and quality in hydroponic cultures,” Agronomy, vol. 11, no. 3, 2021, doi: 10.3390/agronomy11030491.
N. Dhankhar and J. Kumar, “Impact of increasing pesticides and fertilizers on human health: A review,” Mater. Today Proc., 2023, doi: 10.1016/j.matpr.2023.03.766.
M. E. Hossain, S. Shahrukh, and S. A. Hossain, “Chemical fertilizers and pesticides: Impacts on soil degradation, groundwater, and human health in Bangladesh BT-Environmental Degradation: Challenges and Strategies for Mitigation,” V. P. Singh, S. Yadav, K. K. Yadav, and R. N. Yadava, Eds., Cham: Springer International Publishing, 2022, pp. 63–92. doi: 10.1007/978-3-030-95542-7_4.
H. N. Pahalvi, L. Rafiya, S. Rashid, B. Nisar, and A. N. Kamili, “Chemical fertilizers and their impact on soil health BT-Microbiota and Biofertilizers, Vol 2: Ecofriendly Tools for Reclamation of Degraded Soil Environs,” G. H. Dar, R. A. Bhat, M. A. Mehmood, and K. R. Hakeem, Eds., Cham: Springer International Publishing, 2021, pp. 1–20. doi: 10.1007/978-3-030-61010-4_1.
D. Alfarado, S. Shiyan, and F. Ferlinahayati, “Study of Potential α-Glucosidase Inhibitor from Tithonia diversifolia: In Vitro, Pharmacokinetics, Toxicology, and Molecular Docking,” Indones. J. Chem., vol. 25, no. 2, pp. 575–589, 2025, doi: 10.22146/ijc.103111.
U. Karo-Karo et al., “The reduction of armyworms on mustard greens using the extract of Papaya Leaf And Kipahit Leaf,” IOP Conf. Ser. Earth Environ. Sci., vol. 1083, no. 1, 2022, doi: 10.1088/1755-1315/1083/1/012052.
A. Rahayu, N. Rochman, and W. Nahraeni, “Production and Quality of Katuk (Sauropus androgynous (L.) Merr) Plants on Various Composition of Urea Fertilizer and Mexican Sunflower Compost,” J. Hortik. Indones., vol. 12, no. 1, pp. 31–41, 2021, doi: 10.29244/jhi.12.1.31-41.
D. Kurniawan, A. Nadhira, O. S. Sijabat, N. U. Angkat, and R. Rosmaladewi, “Pembuatan POC (Pupuk Organik Cair) dengan menggunakan tanaman kipahit [Making LOF (Liquid Organic Fertilizer) using Kipahit plants],” J. Abdimas Maduma, vol. 2, no. 1, pp. 42–46, 2023, doi: 10.52622/jam.v2i1.152.
T. Endoh, M. Takagaki, P. Suwitchayanon, C. Chanseetis, and N. Lu, “Hydroponic lettuce cultivation with organic liquid fertilizer: Examining bacterial inhibition and phosphate solubilization,” Crops, vol. 4, no. 4, pp. 502–513, 2024, doi: 10.3390/crops4040036.
M. Ezziddine, H. Liltved, and R. Seljåsen, “Hydroponic lettuce cultivation using organic nutrient solution from aerobic digested aquacultural sludge,” Agronomy, vol. 11, no. 8, p. 1484, Jul. 2021, doi: 10.3390/agronomy11081484.
A. Alkaabi et al., “Vertical hydroponic lettuce: Impact of organic nutrients on antioxidant phytochemicals,” Ann. Agric. Sci., vol. 70, no. 1, p. 100386, Jun. 2025, doi: 10.1016/j.aoas.2025.100386.
M. Chowdhury, U. C. Samarakoon, and J. E. Altland, “Evaluation of hydroponic systems for organic lettuce production in controlled environment,” Front. Plant Sci., vol. 15, Aug. 2024, doi: 10.3389/fpls.2024.1401089.
A. M. Ahmed and S. L. Kareem, “Evaluating the role of hydraulic retention time (HRT) in pollutant removal efficiency using Arundo donax in vertical subsurface flow constructed wetlands,” Bioremediat. J., pp. 1–15, Dec. 2024, doi: 10.1080/10889868.2024.2439829.
Y. L. Pang, Y. Y. Quek, S. Lim, and S. H. Shuit, “Review on Phytoremediation Potential of Floating Aquatic Plants for Heavy Metals: A Promising Approach,” Sustain., vol. 15, no. 2, 2023, doi: 10.3390/su15021290.
K. Z. Ansyafa et al., “Intelligent Hydroponic Control System with IoT and Fuzzy Logic on Kale Plants,” J. Appl. Sci. Technol. Humanit., vol. 2, no. 2, pp. 199–222, 2025, doi: 10.62535/5w3gk317.
V. Ghimirey, J. Chaurasia, and S. Marahatta, “Plant nutrition disorders: Insights from clinic analyses and their impact on plant health,” Agric. Ext. Dev. Ctries., no. May, pp. 09–17, 2024, doi: 10.26480/aedc.01.2024.09.17.
X. Jia, A. Speck, X. Feng, and C. W. Lee, “Impact of Water pH and Cultivar on Lettuce Growth, Water Use Efficiency, and Nutrient Use Efficiency in Deep Water Culture Systems,” Water (Switzerland), vol. 17, no. 17, pp. 1–19, 2025, doi: 10.3390/w17172637.
S. S. Yaakup, N. A. Halim, and P. Ding, “Effect of nutrient solution pH on the growth and quality of lactuca sativa grown in a static hydroponic system,” Pertanika J. Trop. Agric. Sci., vol. 47, no. 4, pp. 1175–1189, 2024, doi: 10.47836/pjtas.47.4.08.
A. Balliu, Y. Zheng, G. Sallaku, J. A. Fernández, N. S. Gruda, and Y. Tuzel, “Environmental and cultivation factors affect the morphology, architecture and performance of root systems in soilless grown plants,” Horticulturae, vol. 7, no. 8, 2021, doi: 10.3390/horticulturae7080243.
A. S. Zurano, C. G. Serrano, F. G. Acién-Fernández, J. M. Fernández-Sevilla, and E. Molina-Grima, “Modelling of photosynthesis, respiration, and nutrient yield coefficients in Scenedemus almeriensis culture as a function of nitrogen and phosphorus,” Appl. Microbiol. Biotechnol., vol. 105, no. 19, pp. 7487–7503, 2021, doi: 10.1007/s00253-021-11484-8.
D. Pancawati and A. Yulianto, “Implementasi fuzzy logic controller untuk mengatur Ph Nutrisi pada sistem hidroponik nutrient film technique (NFT) [Implementation of fuzzy logic controller to regulate nutrient pH in nutrient film technique (NFT) hydroponic system],” J. Nas. Tek. Elektro, vol. 5, no. 2, p. 278, 2016, doi: 10.25077/jnte.v5n2.284.2016.
R. Lorrai and S. Ferrari, “Host cell wall damage during pathogen infection: Mechanisms of perception and role in plant-pathogen interactions,” Plants, vol. 10, no. 2, pp. 1–21, 2021, doi: 10.3390/plants10020399.
S. Zhang, C. Li, J. Si, Z. Han, and D. Chen, “Action mechanisms of effectors in Plant‐Pathogen interaction,” Int. J. Mol. Sci., vol. 23, no. 12, 2022, doi: 10.3390/ijms23126758.
N. P. R. Sudiartini, G. Wirya, and I. M. Sudarma, “Identifikasi jamur penyebab penyakit utama pada tanaman selada (Lactuca sativa L.) hidroponik [Identification of the main disease-causing fungi in hydroponic lettuce (Lactuca sativa L.) plants],” J. Agroekoteknologi Trop. ISSN, vol. 2301, p. 6515, 2021.
M. Li et al., “Monitoring by real-time PCR of three water-borne zoosporic Pythium species in potted flower and tomato greenhouses under hydroponic culture systems,” Eur. J. Plant Pathol., 2014.
N. Mahmoud, M. A. H. Abdou, S. Salaheldin, W. S. Soliman, and A. M. Abbas, “The Impact of Irrigation Intervals and NPK/Yeast on the Vegetative Growth Characteristics and Essential Oil Content of Lemongrass,” Horticulturae, vol. 9, no. 3, 2023, doi: 10.3390/horticulturae9030365.
S. Farjana, I. S. Park, and J. M. Choi, “Impact of controlled nitrogen application in water solution on seedling growth, tissue and soil nutrient concentrations in vegetative propagation of strawberry,” Hortic. Environ. Biotechnol., vol. 64, no. 1, pp. 41–50, 2023, doi: 10.1007/s13580-022-00460-4.
B. Ariananda, T. Nopsagiarti, and M. Mashadi, “Pengaruh pemberian berbagai konsentrasi larutan nutrisi AB mix terhadap pertumbuhan dan produksi selada (Lactuca sativa L.) hidroponik sistem floating [The effect of providing various concentrations of AB mix nutrient solution on the growth and production,” Green Swarnadwipa J. Pengemb. ilmu Pertan., vol. 9, no. 2, pp. 185–195, 2020.
Meriaty, A. Sihaloha, and K. D. Pratiwi, “Pertumbuhan dan hasil tanaman selada (Lactuca sativa L.) akibat jenis media tanam hidroponik dan konsentrasi nutrisi ab mix [Growth and yield of lettuce (Lactuca sativa L.) due to the type of hydroponic growing media and concentration of ab mix nutrients],” Agroprimatech, vol. 4, no. 2, pp. 75–84, 2021, doi: 10.34012/agroprimatech.v4i2.1698.
G. V. A. Balansag, E. M. Anud Jr, G. P. A. Balansag, K. M. Anud, and G. N. A. Balansag, “The effect of ab mix nutrient solution on the height, number of leaves and fresh weight of lettuce (Lactuca sativa L.) in hydrophonics cultivation system,” Int. J. Appl. Sci. Res., vol. 06, no. 02, pp. 93–100, 2022, doi: 10.56293/ijasr.2022.5510.
A. En Yulia, M. Murniati, I. R. Dini, and L. Manja, “The effect of combination of AB-mix nutrition with liquid organic fertilizer of tofu liquid waste on hydroponical growth and production of lettage (Lactuca sativa L.),” Int. J. Sci. Res. Arch., vol. 4, no. 1, pp. 165–170, 2021, doi: 10.30574/ijsra.2021.4.1.0190.
J. J. Amrhein, F. Rotondo, C. Kubota, S. A. Miller, and A. L. Testen, “Diagnostic guide for pythium root rot in hydroponic leafy green and herb production,” Plant Heal. Prog., vol. 26, no. 1, pp. 87–95, Jan. 2025, doi: 10.1094/PHP-07-24-0070-DG.
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