Ethnochemical Investigation of Traditional Maize Fermentation Practices in Rural Andean Communities of Peru
Abstract
traditional maize fermentation practices and their underlying chemical processes in Andean communities of Peru using an ethnochemical approach.
Methodology: This study employed an integrated ethnographic and chemical analysis approach, including semi-structured interviews, participant observation, and laboratory analyses using GC-MS, HPLC, and spectrophotometry to evaluate physicochemical parameters and compound profiles.
Main Findings: Results showed a significant decrease in pH (6.8 to 3.9) and an increase in ethanol content during fermentation (p < 0.05). Ethnographic findings revealed structured local knowledge systems that regulate fermentation processes, which were found to correlate with measurable chemical transformations.
Novelty/Originality of this study: This study provides empirical evidence linking cultural fermentation practices with biochemical processes, demonstrating that traditional knowledge systems function as adaptive regulatory mechanisms within fermentation systems.
References
P. B. Thakur and R. P. Thakur, “Exploration of the Indian knowledge system with reference to traditional and modern chemical sciences,” Int. J. Adv. Multidiscip. Res. Educ. Dev., vol. 2, no. 1, pp. 364–367, 2026, doi: https://www.ijamred.com/volume2/issue1/IJAMRED-V2I1P56.pdf.
A. Ridwan, Y. Rahmawati, and A. Mardiah, “Bridging culture and chemistry: Implementing ethnochemistry to enhance chemical literacy of Indonesian high school students,” Multidiscip. Sci. J., vol. 7, no. 11, pp. 1–11, 2025, doi: 10.31893/multiscience.2025530.
B. Rexhepi and A. Bajrami, “Ethno-pedagogical module: A theoretical exploration of knowledge transmission in ethnobiological systems,” Int. J. Environ. Eng. Educ., vol. 7, no. 1, pp. 1–12, 2025, doi: 10.55151/ijeedu.v7i1.181.
M. A. Pratiwi, H. R. Wahyu, and B. E. Normande, “Understanding knowledge acquisition, adaptive strategies, challenges, and preservation methods among traditional fishermen in a digital age,” in BIO Web of Conferences, 2023, pp. 1–12. doi: 10.1051/bioconf/20237005008.
A. Ashari and M. Munawwarah, “Ethnochemistry Supports 21st Century Skills: Systematic Literature Review,” Hydrog. J. Kependidikan Kim., vol. 13, no. 5, pp. 1044–1049, 2025.
A. Zulaika, Erlina, and Rachmat Sahputra, “Ethnochemistry in chemistry learning: Insights from Indonesian local wisdom,” J. Pendidik. MIPA, vol. 26, no. 3, pp. 1642–1658, 2025, doi: 10.23960/jpmipa.v26i3.pp1642-1658.
K. Modi, “Fermenting futures: Food fermentation as an ‘art of noticing,’” J. Posthumanism, vol. 3, no. 3, pp. 269–286, Nov. 2023, doi: 10.33182/joph.v3i3.1344.
G. Sharma and S. K. Biswas, “Food fermentation and preservation among the limbu tribe of the eastern himalayas: An observation,” J. Anthropol. Surv. India, vol. 0, no. 0, pp. 1–19, Feb. 2026, doi: 10.1177/2277436X261415581.
M. López-Reynoso, G. A. Martínez-Medina, L. Londoño-Hernández, P. Aguilar-Zarate, J. U. Hernández-Beltrán, and A. Y. Hernández-Almanza, “Corn-based fermented beverages: Nutritional value, microbial dynamics, and functional potential—an overview,” Foods, vol. 15, no. 1, pp. 1–33, 2026, doi: 10.3390/foods15010027.
C. L. García, F. A. G. Bermúdez, W. Vanden Berghe, M. G. Zurita-Benavides, and A. Orellana-Manzano, “Fermented beverages among indigenous Latin American societies,” Front. Sustain. Food Syst., vol. 8, no. 1390162, pp. 1–19, 2024, doi: 10.3389/fsufs.2024.1390162.
P. O. Ajanaku et al., “Novel fermentation techniques for improving food functionality: An overview,” Fermentation, vol. 11, no. 9, pp. 1–26, 2025, doi: 10.3390/fermentation11090509.
T. Niyigaba, K. Küçükgöz, D. Kołożyn-Krajewska, T. Królikowski, and M. Trząskowska, “Advances in fermentation technology: A focus on health and safety,” Appl. Sci., vol. 15, no. 6, pp. 1–29, 2025, doi: 10.3390/app15063001.
T. J. Hackmann, “The vast landscape of carbohydrate fermentation in prokaryotes,” FEMS Microbiol. Rev., vol. 48, no. 4, pp. 1–18, 2024, doi: 10.1093/femsre/fuae016.
W. Sun, M. H. Shahrajabian, and M. Lin, “Research progress of fermented functional foods and protein factory-microbial fermentation technology,” Fermentation, vol. 8, no. 12, pp. 1–32, 2022, doi: 10.3390/fermentation8120688.
W. Reineke and M. Schlömann, “Microorganisms at Different Sites: Living Conditions and Adaptation Strategies,” in Environmental Microbiology, Berlin, Heidelberg: Springer Berlin Heidelberg, 2023, pp. 349–396. doi: 10.1007/978-3-662-66547-3_10.
P. Kakde and J. Sharma, “Microbial bioremediation of petroleum contaminated soil: Structural complexity, degradation dynamics and advanced remediation techniques,” J. Pure Appl. Microbiol., vol. 18, no. 4, pp. 1–18, 2024, doi: 10.22207/JPAM.18.4.28.
C. K. Anumudu, T. Miri, and H. Onyeaka, “Multifunctional applications of lactic acid bacteria: Enhancing safety, quality, and nutritional value in foods and fermented beverages,” Foods, vol. 13, no. 23, pp. 1–35, 2024, doi: 10.3390/foods13233714.
S. S. Sawant, H. Y. Park, E. Y. Sim, H. S. Kim, and H. S. Choi, “Microbial fermentation in food: Impact on functional properties and nutritional enhancement—a review of recent developments,” Fermentation, vol. 11, no. 1, pp. 1–29, 2025, doi: 10.3390/fermentation11010015.
L. Yuan et al., “Environmental factors at different scales: a review of their effects on spontaneous fermentation Chinese Baijiu and related mechanisms,” Food Sci. Hum. Wellness, vol. 15, pp. 1–13, 2024, doi: 10.26599/fshw.2024.9250339.
B. Peng et al., “Rice wine fermentation: Unveiling key factors shaping quality, flavor, and technological evolution,” Foods, vol. 14, no. 14, pp. 1–23, 2025, doi: 10.3390/foods14142544.
C. E. Bahule, L. H. da S. Martins, B. J. M. Chaúque, and A. S. Lopes, “Metaproteomics as a tool to optimize the maize fermentation process,” Trends Food Sci. Technol., vol. 129, pp. 258–265, Nov. 2022, doi: 10.1016/j.tifs.2022.09.017.
A. S. Samarasinghe, F. Fernando, K. D. Athiyappan, B. Xu, and A. Saeid, “New insights into the use of cereals and pseudocereals in fermented beverages: Trends, challenges, and innovations,” eFood, vol. 6, no. 4, pp. 1–33, 2025, doi: 10.1002/efd2.70089.
Z. Cao, W. Yan, M. Ding, and Y. Yuan, “Construction of microbial consortia for microbial degradation of complex compounds,” Front. Bioeng. Biotechnol., vol. 10, no. December, pp. 1–14, 2022, doi: 10.3389/fbioe.2022.1051233.
R. V. Kapoore, G. Padmaperuma, S. Maneein, and S. Vaidyanathan, “Co-culturing microbial consortia: approaches for applications in biomanufacturing and bioprocessing,” Crit. Rev. Biotechnol., vol. 42, no. 1, pp. 46–72, 2022, doi: 10.1080/07388551.2021.1921691.
R. V. Bacter et al., “From heritage to modern economy: Quantitative surveys and ethnographic insights on sustainability of traditional bihor products,” Agric., vol. 15, no. 13, pp. 1–28, 2025, doi: 10.3390/agriculture15131404.
P. Silva-Ávila, J. Rojas Hernández, and R. O. Barra, “Knowledge alliances for global change adaptation: A relational approach based on traditional ecological knowledge, territorial management, and community practices in the chilean context,” Sustain., vol. 17, no. 8, pp. 1–28, 2025, doi: 10.3390/su17083653.
S. P. Chand, “Methods of data collection in qualitative research: Interviews, focus groups, observations, and document analysis,” Adv. Educ. Res. Eval., vol. 6, no. 1, pp. 303–317, 2025, doi: 10.25082/aere.2025.01.001.
J. Ominyi, U. Eze, D. Agom, A. Alabi, and A. Nwedu, “Implementing evidence-based practice in critical care nursing: An ethnographic case study of knowledge use,” J. Adv. Nurs., vol. 82, pp. 2407–2426, 2026, doi: 10.1111/jan.70054.
J. Forsberg, C. T. Rasmussen, F. W. J. van den Berg, S. B. Engelsen, and V. Aru, “Fermentation Analytical Technology (FAT): Monitoring industrial E. coli fermentations using absolute quantitative 1H NMR spectroscopy,” Anal. Chim. Acta, vol. 1311, no. 342722 Contents, pp. 1–10, 2024, doi: 10.1016/j.aca.2024.342722.
C. S. Yee et al., “Smart fermentation technologies: Microbial process control in traditional fermented foods,” Fermentation, vol. 11, no. 6, pp. 1–38, 2025, doi: 10.3390/fermentation11060323.
M. E. Jimenez, C. M. O’Donovan, M. F. de Ullivarri, and P. D. Cotter, “Microorganisms present in artisanal fermented food from South America,” Front. Microbiol., vol. 13, no. September, pp. 1–18, 2022, doi: 10.3389/fmicb.2022.941866.
L. S. Guerra, J. M. Cevallos-Cevallos, S. Weckx, and J. Ruales, “Traditional fermented foods from ecuador: A review with a focus on microbial diversity,” Foods, vol. 11, no. 13, pp. 1–14, 2022, doi: 10.3390/foods11131854.
S. Schechtel and A. Bongers, “Representing chemistry culture: ethnography’s methodological potential in chemistry education research and practice,” Chem. Educ. Res. Pract., vol. 25, no. 3, pp. 584–593, 2024, doi: 10.1039/D3RP00272A.
E. U. Alum et al., “Metabolomics-driven standardization of herbal medicine: Advances, applications, and sustainability considerations,” Nat. Prod. Commun., vol. 20, no. 8, pp. 1–16, 2025, doi: 10.1177/1934578X251367650.
P. Du, R. Fan, N. Zhang, C. Wu, and Y. Zhang, “Advances in integrated multi-omics analysis for drug-target identification,” Biomolecules, vol. 14, no. 6, pp. 1–25, 2024, doi: 10.3390/biom14060692.
A. Morabito, G. De Simone, R. Pastorelli, L. Brunelli, and M. Ferrario, “Algorithms and tools for data-driven omics integration to achieve multilayer biological insights: a narrative review,” J. Transl. Med., vol. 23, no. 1, pp. 1–26, 2025, doi: 10.1186/s12967-025-06446-x.
Y. M. R. Muhammed, F. Minervini, and I. Cavoski, “From ancient fermentations to modern biotechnology: Historical evolution, microbial mechanisms, and the role of natural and commercial starter cultures in shaping organic and sustainable food systems,” Foods, vol. 14, no. 24, pp. 1–36, 2025, doi: 10.3390/foods14244240.
V. Tripathi, “Analyzing the role of legal protection for trademarks and geographical indications in preserving cultural heritage and enhancing global trade,” J. Law Intellect. Prop. Rights, vol. 1, no. 1, pp. 50–63, 2024, [Online]. Available: www.ciir.in
D. A. Teferi et al., “Tella (Ethiopian traditional beer): brewing, microbiology, nutrition, health implications, byproducts, and challenges,” Cogent Food Agric., vol. 12, no. 1, pp. 1–36, 2026, doi: 10.1080/23311932.2026.2631822.
J. P. Tamang, “Dietary culture and antiquity of the Himalayan fermented foods and alcoholic fermented beverages,” J. Ethn. Foods, vol. 9, no. 1, pp. 1–18, 2022, doi: 10.1186/s42779-022-00146-3.
M. Kurtkoti and P. Joshi, “Gender and tribal knowledge systems : Women’s role in preserving indigenous knowledge,” Young Res., vol. 13, no. 1, pp. 20–33, 2024.
L. T. Baniaga, “Challenges and opportunities in integrating indigenous culinary practices into the technology and livelihood education curriculum,” Stud. Interdiscip. Horizons, vol. 1, no. 3, pp. 18–27, 2025, doi: 10.64358/z4jvyc05.
L. Liu et al., “The fermentation law of biogenic amines in the pre-fermentation process is revealed by correlation analysis,” Foods, vol. 14, no. 4, pp. 1–15, 2025, doi: 10.3390/foods14040583.
W. Wang et al., “Changes in vinegar quality and microbial dynamics during fermentation using a self-designed drum-type bioreactor,” Front. Nutr., vol. 10, no. 1126562, pp. 1–12, 2023, doi: 10.3389/fnut.2023.1126562.
Y. Wang et al., “Visualizing chemical indicators: Spatial and temporal quality formation and distribution during black tea fermentation,” Food Chem., vol. 401, p. 134090, Feb. 2023, doi: 10.1016/j.foodchem.2022.134090.
H. Shi et al., “Dynamic changes in the chemical composition and metabolite profiles of drumstick (Moringa oleifera Lam.) leaf flour during fermentation,” Lwt, vol. 155, p. 112973, 2022, doi: 10.1016/j.lwt.2021.112973.
P. Burawat and P. Peamchai, “Investment feasibility analysis for pasteurized bottled fermented fish sauce production in Thailand: perspectives from industry stakeholders,” Int. J. Syst. Assur. Eng. Manag., vol. 16, no. 8, pp. 2767–2784, Aug. 2025, doi: 10.1007/s13198-025-02837-x.
A. Albaiti, L. Narsia, M. Gultom, and F. Deminggus, “The bridge of tradition and learning science : Mapping rthnochemical mental models based on the sasisen and Napnap Mor traditions of the Biak ethnic, Papua, Indonesia,” J. Cult. Values Educ., vol. 9, no. 1, pp. 269–295, 2026, doi: 10.46303/jcve.2026.12 How.
F. J. García-Peñalvo, “Developing robust state-of-the-art reports: Systematic Literature Reviews,” Educ. Knowl. Soc., vol. 23, p. E28600, 2022, doi: 10.14201/eks.28600.
J. He, C. Treude, and D. Lo, “LLM-based multi-agent systems for software engineering: Literature review, vision, and the road ahead,” ACM Trans. Softw. Eng. Methodol., vol. 34, no. 5, pp. 1–30, Jun. 2025, doi: 10.1145/3712003.
S. R. Maji, C. Roy, and S. K. Sinha, “Gas chromatography-mass spectrometry (GC-MS): a comprehensive review of synergistic combinations and their applications in the past two decades,” J. Anal. Sci. Appl. Biotechnol., vol. 5, no. 2, pp. 72–85, 2023, doi: 10.48402/IMIST.PRSM/jasab-v5i2.40209.
Y. He et al., “Characterization of key compounds of organic acids and aroma volatiles in fruits of different actinidia argute resources based on high-performance liquid chromatography (HPLC) and headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS),” Foods, vol. 12, no. 19, pp. 1–28, 2023, doi: 10.3390/foods12193615.
V. D. Prokopiou, A. Karampatea, Z. S. Metaxa, and A. V Tsoupras, “Biosensors of wine fermentation for monitoring chemical and biochemical interactions, process indicators and migration of compounds and metabolites , between wine and fermentation vessels — a critical review,” Biosensors, vol. 16, no. 153, pp. 1–58, 2026, doi: 10.3390/bios16030153.
D. K. Yadav, K. Chand, and P. Kumari, “Effect of fermentation parameters on physicochemical and sensory properties of Burans wine,” Syst. Microbiol. Biomanufacturing, vol. 2, no. 2, pp. 380–392, Apr. 2022, doi: 10.1007/s43393-021-00074-4.
F. G. Nazhira, “Barriers to implementing organic waste-based fermented feed practices among livestock farmers in Kuningan, West Java,” Livest. Sci. Innov. J., vol. 2, no. 2, pp. 66–90, 2025, doi: 10.59261/lsij.v2i2.29.
S. P. Chand, “Methods of Data Collection in Qualitative Research: Interviews, Focus Groups, Observations, and Document Analysis,” Adv. Educ. Res. Eval., vol. 6, no. 1, pp. 303–317, Aug. 2025, doi: 10.25082/AERE.2025.01.001.
C. L. Arntson and M. N. Yoon, “Participant directed mobile interviews: A data collection method for conducting in-situ field research at a distance,” Int. J. Qual. Methods, vol. 22, pp. 1–9, 2023, doi: 10.1177/16094069231188254.
D. C. Tura, “Ethnographic qualitative study to explore the sociocultural values, nutritional potential, and health benefits of dabi Teff (Eragrostis Tef) grown in Western Ethiopia,” Food Sci. Nutr., vol. 13, no. 11, pp. 1–13, 2025, doi: 10.1002/fsn3.71130.
M. F. Fiadillah and W. Sumarni, “Science in samin: Reconstructing indigenous knowledge of the samin community into chemical concepts for contextualized education,” J. Innov. Educ. Cult. Res., vol. 7, no. 1, pp. 191–205, 2026, doi: 10.46843/jiecr.v7i1.2593.
B. Chibuye and I. Sen Singh, “Integration of local knowledge in the secondary school chemistry curriculum - A few examples of ethno-chemistry from Zambia,” Heliyon, vol. 10, no. 7, pp. 1–15, 2024, doi: 10.1016/j.heliyon.2024.e29174.
M. D. Astuti, H. Hendrawani, and K. Khaeruman, “Integrating Sukarara traditional weaving into chemistry education: Impact on students’ cultural literacy and critical thinking skills,” Hydrog. J. Kependidikan Kim., vol. 14, no. 1, pp. 7–14, 2026, doi: 10.33394/hjkk.v14i1.19331.
A. B. Ndeko et al., “Farmers’ preferred traits, production constraints, and adoption factors of improved maize varieties under South-Kivu rainfed agro-ecologies, eastern D.R. Congo: implications for maize breeding,” Int. J. Agric. Sustain., vol. 23, no. 1, pp. 1–26, 2025, doi: 10.1080/14735903.2025.2464524.
J. R. Bayoï and W. Dieudonné, “Traditional production practices, nutraceutical potential, safety evaluation, and chemometric analysis of furdu sorghum beer: A comprehensive study across dix divisions in far north cameroon,” Int. J. Food Sci., vol. 2026, no. 1, pp. 1–19, Jan. 2026, doi: 10.1155/ijfo/8898315.
M. M. Sooresh, B. P. Willing, and B. C. T. Bourrie, “Opportunities and challenges of understanding community assembly in spontaneous food fermentation,” Foods, vol. 12, no. 3, pp. 1–16, 2023, doi: 10.3390/foods12030673.
I. Izquierdo-Bueno, J. Moraga, J. M. Cantoral, M. Carbú, C. Garrido, and V. E. González-Rodríguez, “Smart viniculture: Applying artificial intelligence for improved winemaking and risk management,” Appl. Sci., vol. 14, no. 22, pp. 1–32, 2024, doi: 10.3390/app142210277.
Y. H. Du, M. Y. Wang, L. H. Yang, L. L. Tong, D. S. Guo, and X. J. Ji, “Optimization and scale-up of fermentation processes driven by models,” Bioengineering, vol. 9, no. 9, pp. 1–18, 2022, doi: 10.3390/bioengineering9090473.
Z. Yao, T. Xie, H. Deng, S. Xiao, and T. Yang, “Directed evolution of microbial communities in fermented foods: strategies, mechanisms, and challenges,” Foods, vol. 14, no. 2, pp. 1–22, 2025, doi: 10.3390/foods14020216.
Q. Du, D. Ye, X. Zang, H. Nan, and Y. Liu, “Effect of low temperature on the shaping of yeast-derived metabolite compositions during wine fermentation,” Food Res. Int., vol. 162, p. 112016, Dec. 2022, doi: 10.1016/j.foodres.2022.112016.
C. X. Thuy et al., “Effect of fermentation conditions (dilution ratio, medium pH, total soluble solids, and saccharomyces cerevisiae yeast ratio) on the ability to ferment cider from tamarillo (solanum betaceum) fruit,” J. Food Process. Preserv., vol. 2024, pp. 1–17, 2024, doi: 10.1155/2024/8841207.
N. Aramrueang, P. Lomwongsopon, S. Boonsong, and P. Kingklao, “Improved spectrophotometric method for determination of high-range volatile fatty acids in mixed acid fermentation of organic residues,” Fermentation, vol. 8, no. 5, pp. 1–17, 2022, doi: 10.3390/fermentation8050202.
G. Galarza and J. G. Figueroa, “Volatile compound characterization of coffee (Coffea arabica) processed at different fermentation times using SPME–GC–MS,” Molecules, vol. 27, no. 6, pp. 1–15, 2022, doi: 10.3390/molecules27062004.
R. A. R. Rocha et al., “Evaluation of arabica coffee fermentation using machine learning,” Foods, vol. 13, no. 3, pp. 1–18, 2024, doi: 10.3390/foods13030454.
J. Sung and J. Cheong, “Quantum medicine: A quantum–mechanical framework for redox biology, disease and precision medicine,” Clin. Transl. Med., vol. 16, no. 1, pp. 1–24, 2026, doi: 10.1002/ctm2.70598.
U. Wyne, “Neuro-spirituality: A brain-based framework for redefining consciousness and spiritual experience,” Adv. Soc. Sci. Arch. J., vol. 5, no. 1, pp. 2255–2265, 2026, [Online]. Available: https://www.preventionweb.net/news/preliminary-report-february-6-2023-earthquakes-turkiye
C. Su, Y. Han, X. Tang, Q. Jiang, T. Wang, and Q. He, “Knowledge-based digital twin system: Using a knowlege-driven approach for manufacturing process modeling,” Comput. Ind., vol. 159–160, p. 104101, Aug. 2024, doi: 10.1016/j.compind.2024.104101.
P. Brauner et al., “A computer science perspective on digital transformation in production,” ACM Trans. Internet Things, vol. 3, no. 2, pp. 1–32, May 2022, doi: 10.1145/3502265.
K. Zhang et al., “Water quality impact on fish behavior: A review from an aquaculture perspective,” Rev. Aquac., vol. 17, no. 1, pp. 1–27, 2025, doi: 10.1111/raq.12985.
X. Tang, C. Lu, P. Meng, and W. Cheng, “Spatiotemporal evolution of the environmental adaptability efficiency of the agricultural system in China,” Sustain., vol. 14, no. 6, pp. 1–15, 2022, doi: 10.3390/su14063685.
Z. T. Al-Sharify et al., “Optimizing beverage production: The role of fluid dynamics in microbial fermentation,” J. Food Process. Preserv., vol. 2025, no. 1, pp. 1–15, 2025, doi: 10.1155/jfpp/8811731.
V. T. Herlina, R. H. B. Setiarto, and I. B. A. Yogeswara, “Unveiling the sociocultural, microbiological, and functional properties of liquid brem, a traditional balinese fermented rice wine: a systematic review,” J. Ethn. Foods, vol. 13, no. 4, pp. 1–17, 2026, doi: 10.1186/s42779-026-00305-w.
Y. Liu, L. Luo, and L. Zeng, “Guidelines for sensory evaluation of tea: Traditional chinese method and quantitative descriptive analysis,” AgriFood J. Agric. Prod. Food, vol. 1, no. 1, pp. 15–20, 2025, doi: 10.1002/agf2.70001.
M. Y. B. Adjei et al., “Integrative review on the use of sensory evaluation methods in consumer-led product development for indigenous fruits and vegetables,” Front. Sustain. Food Syst., vol. 9, no. January, pp. 1–12, 2026, doi: 10.3389/fsufs.2025.1657001.
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