Evaluation of Curcumin as NF-κB Inhibitor for Breast Cancer Therapy Using In Silico Approach

  • Endang Adillah Alauddin State Islamic University of Makassar
  • Nour Albahri Tishreen University
  • Shrouk Ekramy Al Azhar University
DOI: https://doi.org/10.37251/jouabe.v2i1.1947
Keywords: Breast Cancer, Curcumin, In Silico Analysis, Molecular Docking, NF-κB

Abstract

Purpose of the study: This study aims to evaluate the potential of curcumin as a breast cancer therapeutic agent by analyzing its ability to inhibit NF-κB activation using an in silico approach, specifically through molecular docking to assess absorption and interaction with the target protein.

Methodology: This study used a quantitative experimental method with molecular docking as an in silico approach. Tools included a Lenovo laptop (Intel® Inside™, Windows 10 Pro). Software used: PyRx 0.8 (with AutoDock Vina), YASARA, PyMOL, and Discovery Studio 2019. Ligand data were obtained from PubChem; proteins from Protein Data Bank. Lipinski's Rule was applied for drug-likeness screening.

Main Findings: Curcumin showed a binding affinity of -6.2 kcal/mol to NF-κB with RMSD < 2.00 Å. Visualization confirmed hydrogen bonds at ASN 32 and ASN 47, and hydrophobic pi-alkyl interactions at ALA 34 and ARG 50. Lipinski's Rule of Five was fulfilled, indicating good oral drug-likeness and potential as an NF-κB inhibitor in breast cancer therapy.

Novelty/Originality of this study: This study offers new insights into the potential of curcumin as a natural NF-κB inhibitor for breast cancer therapy through a comprehensive in silico approach. By combining molecular docking, visualization, and drug-likeness analysis, it advances current knowledge by highlighting curcumin's binding efficiency and pharmacological feasibility, supporting its development as an alternative anticancer candidate.

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Published
2025-07-03
How to Cite
Adillah, E., Albahri, N., & Ekramy, S. (2025). Evaluation of Curcumin as NF-κB Inhibitor for Breast Cancer Therapy Using In Silico Approach. Journal of Academic Biology and Biology Education, 2(1). https://doi.org/10.37251/jouabe.v2i1.1947
Issue
Vol. 2 No. 1 (2025): June
Section
Articles

Copyright (c) 2025 Endang Adillah, Nour Albahri, Shrouk Ekramy

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