Integrated Science Education Journal
Integrated Science Education Journal

an Open Access Journal

SINTA

6.1

Impact

GScholar

18

H-Index

Integrated Science Education Journal

an Open Access Journal


Microcapsule Formulation of Pumpkin Seeds (Cucurbita moschata) as a Potential Supplement to Support Iron and Zinc Adequacy in Stunting Prevention

Share
  • Purpose of the study: Iron (Fe) and zinc (Zn) deficiencies significantly contribute to childhood stunting. Pumpkin seeds (Cucurbita moschata) are rich in these minerals, but poor palatability and rapid gastric degradation limit their direct use as a food ingredient. This study aimed to microencapsulate pumpkin seed powder with gum arabic via solvent evaporation to protect key micronutrients and enhance targeted mineral delivery.

    Methodology: The prepared experimental samples were characterized using PSA, FTIR, AAS, and a binocular microscope. We formulated microcapsules with a high-speed homogenizer using the solvent evaporation technique, and statistically analyzed the data using ANOVA in SPSS.

    Main Findings: The synthesized pumpkin seed microcapsules were powder-shaped, yellow, aromatic, and tasteless. Formulas ranging from 1.5:5 to 3.5:5 were the most stable through the sixth cycle. The yield (38.8%–77%), particle size (54.68–60.70 μm), and particle uniformity were optimal at a 4.5:5 ratio, exhibiting round morphological shapes. Mineral concentrations reached 18–101 mg/L for Fe and 11–79 mg/L for Zn, with encapsulation efficiency between 2.81% and 30.01%. FTIR spectra showed characteristic peaks from both raw materials, and microbiological testing confirmed safety under official BPOM standards.

    Novelty/Originality of this study: Increasing the active ingredient ratio significantly enhanced mineral uptake without altering the fundamental chemical structure. The primary novelty lies in utilizing pumpkin seed waste encapsulated in gum arabic via solvent evaporation. These stable microcapsules represent a safe, effective, and economical local food fortification agent to address stunting.

  • How to cite

    [1]
    “Microcapsule Formulation of Pumpkin Seeds (Cucurbita moschata) as a Potential Supplement to Support Iron and Zinc Adequacy in Stunting Prevention”, In. Sci. Ed. J, vol. 7, no. 5, pp. 928–940, Sep. 2026, doi: 10.37251/isej.v7i5.3745.
  • 7
    Abstract views
    1
    Downloads

    Metrics — Badges

    1. [1] Kementerian Kesehatan Republik Indonesia, “Hasil Survei Status Gizi Indonesia (SSGI) Tahun 2024 [Results of the Indonesian Nutritional Status Survey (SSGI) 2024],” Badan Kebijakan Pembangunan Kesehatan, Kemenkes RI, 2024, https://www.badankebijakan.kemkes.go.id.
    2. [2] A. Mulyana, Hendra, and Retnoningsih, “Implikasi stunting terhadap kemampuan kognitif dan fisik anak usia dini di tk at-taqwa teta lambitu [Implications of Stunting on the Cognitive and Physical Abilities of Early Childhood at At-Taqwa Teta Lambitu Kindergarten],” Pendas: Jurnal Ilmiah Pendidikan Dasar, vol. 10, no. 02, pp. 110–121, 2025, doi: 10.23969/jp.v10i02.24512.
    3. [3] A. Syam, F. K. Burhan, V. Hadju, Citrakesumasari, and A. M. Akhmar, “The effect of biscuits made from pumpkin seeds flour on serum zinc levels and weight in malnutrition wistar rats,” Open Access Macedonian Journal of Medical Sciences, vol. 8, no. A, pp. 428–433, 2020, doi: 10.3889/oamjms.2020.4402.
    4. [4] R. Rahmadevi, S. M. Lestari, I. M. N. Khasanah, and C. W. Putri, “Lempuyang Rhizome Extract Microcapsule Formulation (Zingiber zerumbet) For Enhancer Appetite in Children,” Riset Informasi Kesehatan, vol. 12, no. 2, pp. 192–197, 2023, doi: 10.30644/rik.v12i2.824.
    5. [5] A. R. Erwiyani, I. Sunnah, and N. M. Pratama, “Formulation and Evaluation of Microcapsul of Pumpkin (Cucurbita maxima D.) Extract Peel Off Gel Mask,” Indonesian Journal of Pharmaceutical Science and Technology, vol. 11, no. 3, pp. 345–355, 2024, doi: 10.24198/ijpst.v11i3.36986.
    6. [6] T. A. Khasanah, and C. E. Mumpuni, “Pengaruh formulasi tepung ikan haruan, tepung buah dan biji labu kuning pada biskuit terhadap kandungan gizi dan daya terima [Effect of haruan fish flour, yellow pumpkin fruit and seed flour formulations in biscuits on nutritional content and acceptance],” Journal of Nutrition College, vol. 10, no. 1, pp. 1-9, 2021, doi: 10.14710/jnc.v10i1.28486.
    7. [7] R. Rahmadevi, C. Khutami, and N. Oktavia, “Formulasi nanokapsul biji labu (Cucurbita moschata) sebagai suplemen zat besi dan zink untuk stunting [Nanokapsul formulation of turb (Cucurbita moschata) turb test as a supplement of iron and zink for stunting],” Jurnal Kesehatan Medika Saintika, vol. 15, no. 2, pp. 315–322, 2024, doi: 10.30633/jkms.v15i2.2950.
    8. [8] A. Hussain, “Development of nutritional biscuits for children, rich in Fe and Zn, by incorporation of pumpkin (Cucurbita maxima) Seeds powder; a healthy pharma food in current post COVID 19 Period,” Pure and Applied Biology, vol. 12, no. 1, pp. 392–403, 2023, doi: 10.19045/bspab.2023.120042.
    9. [9] J. M. Dotto and J. S. Chacha, “The Potential Of Pumpkin Seeds as A Functional Food Ingredient: A review,” Scientific African, vol. 10, pp. 00575, 2020, doi: 10.1016/j.sciaf.2020.e00575.
    10. [10] N. R. Hidayati, “Peningkatan nilai fungsional protein biji labu kuning (Cucurbita moschata) melalui teknologi enkapsulasi: artikel review [Enhancing the functional value of pumpkin seed protein (Cucurbita moschata) through encapsulation technology: A review article],” Jurnal Kolaboratif Sains, vol. 9, no. 2, pp. 2106–2215, 2026, doi: 10.56338/jks.v9i2.9696.
    11. [11] H. Rosi and D. Farmasi, “Mikroenkapsulasi parasetamol menggunakan eudragit l100 sebagai penyalut [Microencapsulation of paracetamol using eudragit l100 as coating],” SITAWA: Jurnal Sains dan Obat Tradisional, vol. 1, no. 2, pp. 92–100, 2022, doi: 10.62018/sitawa.v1i2.16.
    12. [12] F. R. Gunawan, “Mikroenkapsulasi berbasis gum arabik dari ekstrak air pletekan (Ruellia tuberosa L.) dan aktivitas inhibisi terhadap alpha amilase [Arabic gum based microencapsulation of pletekan (Ruellia tuberosa L.) water extract and its inhibitory activity against alpha amylase],” The Indonesian Green Technology Journal, vol. 11, no. 02, pp. 79–85, 2022, doi: 10.21776/ub.igtj.2022.011.02.05.
    13. [13] S. Dahlizar et al., “Mikroenkapsulasi daun pala menggunakan maltodekstrin dan gom arab dengan teknik spray drying [Microencapsulation of nutmeg leaves using maltodextrin and gum arabic via spray drying technique],” Pharmaceutical and Biomedical Sciences Journal (PBSJ), vol. 8, no. 1, pp. 10–20, 2026, doi: 10.15408/pbsj.v8i1.46181.
    14. [14] E. Neswita, R. Lubis, F. A. Tanjung, C. E. Novita, A. P. F. Sinaga, and B. Samin, “Mikroenkapsulasi atenolol dengan penyalut hidroksipropil metilselulosa (HPMC) menggunakan metode emulsifikasi penguapan pelarut [Microencapsulation of atenolol with hydroxypropyl methylcellulose (HPMC) coating using the solvent evaporation emulsification method],” Jurnal Prima Medika Sains, vol. 4, no. 1, pp. 21–26, 2022, doi: 10.34012/jpms.v4i1.2261.
    15. [15] H. Ratih, G. Kamilah Pratiwi, F. Alatas, M. Agustin, and B. D. Saraswati, “Isoniazid Microencapsulation with HPMC HP-50 and HPMC HP-55 (2:3) Coating Using Solvent Evaporation Methods,” Indonesian Journal of Pharmaceutical Science and Technology, vol. 9, no. 2, pp. 117-125, 2022, doi: 10.24198/ijpst.v9i2.36513.
    16. [16] I. Brlek, A. Ludaš, and A. Sutlović, "Synthesis And Spectrophotometric Analysis Of Microcapsules Containing Immortelle Essential Oil," Molecules, vol. 26, no. 8, pp. 2390, 2021, doi: 10.3390/molecules26082390.
    17. [17] M. V. Loureiro, A. Aguiar, R. G. dos Santos, J. C. Bordado, I. Pinho, and A. C. Marques, “Design of experiment for optimizing microencapsulation by the solvent evaporation technique,” Polymers, vol. 16, no. 1, pp. 111, 2024, doi: 10.3390/polym16010111.
    18. [18] Sitti et al., “Kalibrasi dosimeter saku gamma meggunakan sumber radioaktif cesium-137 di balai pengamanan fasilitas kesehatan (BPFK) Makassar [Calibration of gamma pocket dosimeter using cesium-137 radioactive source at health facility protection center (BPFK) Makassar],” Jurnal Sains Fisika: SAINFIS, vol. 3, no. 1, pp. 50–59, 2023, doi: 10.24252/sainfis.v3i1.35579.
    19. [19] T. Hasan, M. Agung Irawan, A. P. Tulak, M. Resky Pratama, and E. Dwijayanti, “Sosialisasi pentingnya perawatan dan kalibrasi berkala peralatan instrumen di laboratorium pertambangan [Socialization of the importance of maintenance and periodic calibration of instrument equipment in mining laboratories],” PROFICIO: Jurnal Pengabdian Kepada Masyarakat, vol. 7, no. 1, pp. 984–987, 2026, doi: 10.36728/jpf.v7i1.6041.
    20. [20] A. Jesse Andy Firdaus, D. Pramono, and W. Purnomo, “Pengembangan sistem informasi upt kalibrasi dinas kesehatan kabupaten malang berbasis web [Web based information system development of calibration UPT at Malang regency health office],” Jurnal Sistem Integrasi, Pendidikan, dan Sistem Informasi, vol. 1, no. 1, pp. 23–34, 2020, doi: 10.25126/justsi.v1i1.3.
    21. [21] S. Sugito, S. D. Marliyana, and H. D. Apriana, “Uji kinerja instrumen spektrofotometer serapan atom (aas) shimadzu 6650 f terhadap logam berat [Performance test of atomic absorption spectrophotometer (aas) instrument shimadzu 6650 f against heavy metals],” Journal Of Laboratory, vol. 5, no. 2, pp. 83, 2022, doi: 10.22146/ijl.v5i2.75876.
    22. [22] F. C. Kusumasari, “Pengaruh perbandingan obat-polimer terhadap karakteristik fisik mikrokapsul simvastatin [The effect of drug polymer ratio on the physical characteristics of simvastatin microcapsules],” Jurnal Mandala Pharmacon Indonesia, vol. 9, no. 1, pp. 111–118, 2023, doi: 10.35311/jmpi.v9i1.316.
    23. [23] D. Arziyah, L. Yusmita, and R. Wijayanti, “Analisis mutu organoleptik sirup kayu manis dengan modifikasi perbandingan konsentrasi gula [Organoleptic quality analysis of cinnamon syrup with modification of sugar concentration ratio],” Jurnal Penelitian Dan Pengkajian Ilmiah Eksakta, vol. 1, no. 2, pp. 105–109, 2022, doi: 10.47233/jppie.v1i2.602.
    24. [24] E. Julaeha, S. Puspita, T. Wahyudi, J. Nugraha, and D. R. Eddy, “Mikroenkapsulasi minyak asiri jeruk nipis dengan koaservasi kompleks yang beraktivitas antibakteri [Microencapsulation of lime essential oil by complex coacervation with antibacterial activity],” Arena Tekstil, vol. 35, no. 2, pp. 67–76, 2020, doi: 10.31266/at.v35i2.6607.
    25. [25] F. Rexhepi et al., “Chemical changes of pumpkin seed oils and the impact on lipid stability during thermal treatment: study by FTIR spectroscopy,” Journal of Microbiology, Biotechnology and Food Sciences, vol. 11, no. 6, pp. 5839, 2022, doi: 10.55251/jmbfs.5839.
    26. [26] A. W. Indrianingsih, W. Apriyana, V. T. Rosyida, K. Nisa, S. Nurhayati, and C. Darsih, “Efek pengeringan pada bagian buah labu kuning terhadap aktivitas antioksidan dan struktur morfologi [Drying effect on pumpkin fruit parts on antioxidant activity and morphological structure],” Jurnal Riset Teknologi Industri, vol. 13, no. 1, pp. 12–22, 2019, doi: 10.26578/jrti.v13i1.4395.
    27. [27] Sugito and S. D. Marliyana, “Uji performa spektrofotometer serapan atom thermo ice 3000 terhadap logam pb menggunakan CRM [Performance test of atomic absorption spectrophotometer thermo ice 3000 on pb metal using CRM],” Journal Of Laboratory, vol. 4, no. 2, pp. 67-71, 2021, doi: 10.22146/ijl.v4i2.67438.
    28. [28] M. A. Said, R. W. Utami, and A. Khumaira, “Uji angka lempeng total (ALT) dan angka kapang khamir (AKK) simplisia kunyit (Curcuma domestica) [Total Plate Count (TPC) and Yeast Mold Count (YMC) Test of Turmeric Simplicia (Curcuma domestica)]”, Pro. Senmas. LPPM. Unisa. Yogya, vol. 1, pp. 231–236, 2023.
    29. [29] A. Fatiqin, R. Novita, and I. Apriani, “Pengujian Salmonella dengan menggunakan media SSA dan E. Coli menggunakan media EMBA pada bahan pangan [Testing Salmonella Using SSA Media and E. Coli Using EMBA Media in Food Products]," Jurnal Indobiosains, vol. 1, no. 1, pp. 15–22, 2019, doi: 10.31851/indobiosains.v1i1.2206.
    30. [30] S. Wahidah, G. A. R. Saputri, and N. Nofita, “Formulasi dan uji stabilitas sediaan gel ekstrak etanol daun asam jawa dengan variasi gelling agent [Formulation and stability test of tamarind leaf ethanol extract gel preparations with gelling agent variations],” Jurnal Mandala Pharmacon Indonesia, vol. 10, no. 2, pp. 508–518, 2024, doi: 10.35311/jmpi.v10i2.623.
    31. [31] C. M. Erawati and A. D. R. Dewi, “Optimization of high protein and zinc composite flour formulation with response surface methodology,” Jurnal Teknologi Dan Industri Pangan, vol. 33, no. 2, pp. 119–128, 2022, doi: 10.6066/jtip.2022.33.2.119.
    32. [32] D. Kameliani and M. Putri, “Formulasi dan evaluasi fisik suspensi topikal ekstrak daun kelor dalam pengembangan sediaan antijamur [Formulation and physical evaluation of moringa leaf extract topical suspension in antifungal preparation development],” JIKAS, vol. 4, no. 1, pp. 15–27, 2026, doi: 10.59024/jikas.v4i1.1764.
    33. [33] R. Z. Rahadyana, K. S. Artini, and T. S. Wardani, “Uji aktivitas antioksidan ekstrak biji bunga matahari dengan menggunakan metode DPPH [Activity test of sunflower seed extract using DPPH method],” Jurnal Kesehatan Tambusai, vol. 5, no. 3, pp. 8049–8056, 2024, doi: 10.31004/jkt.v5i3.33567.
    34. [34] E. Sulastri, N. Ibrahim, and S. Budiarti, “Mikroenkapsulasi likopen dari buah tomat dengan metode penguapan pelarut [Microencapsulation of lycopene from tomato fruit using solvent evaporation method],” Jurnal Farmasi Galenika, vol. 5, no. 1, pp. 108–116, 2019, doi: 10.22487/j24428744.2019.v5.i1.12406.
    35. [35] F. Hulu, R. Razoki, and R. Ikhtiari, “Evaluasi formulasi sediaan mikroenkapsulasi ekstrak etanol daun mengkudu [Evaluation of microencapsulated formulations of noni leaf ethanol extract],” Journal of Pharmaceutical and Sciences, vol. 9, no. 2, pp. 1485–1492, 2026, doi: 10.36490/journal-jps.com.v9i2.1574.
    36. [36] M. Nisa et al., “Mikroenkapsulasi ekstrak buah buni menggunakan maltodekstrin dengan metode spray drying [Microencapsulation of buni fruit extract using maltodextrin by spray drying method],” Jurnal Ilmiah Ibnu Sina, vol. 4, no. 2, pp. 285–294, 2019, doi: 10.36387/jiis.v4i2.270.
    37. [37] S. A. Mardikasari, Suryani, N. I. Akib, and R. Indahyani, “Mikroenkapsulasi asam mefenamat menggunakan polimer kitosan dan natrium alginat [Microencapsulation of mefenamic acid using chitosan and sodium alginate polymers],” Jurnal Farmasi Galenika, vol. 6, no. 2, pp. 192–203, 2020, doi: 10.22487/j24428744.2020.v6.i2.14589.
    38. [38] Y. E. Christian and R. Suhardin, “Evaluasi formulasi mikrokapsul transfersom ekstrak etanol 96% jahe merah [Evalution of transfersome microcapsules formulation of 96% red ginger ethanol extract],” Journal of Pharmaceutical Science and Clinical Research, vol. 10, no. 3, pp. 332–352, 2025, doi: 10.20961/jpscr.v10i39801.
    39. [39] M. S. Khuddin, R. A. Karimah, N. Z. Ummah, and K. Maghfiroh, “Mikroenkapsulasi ekstrak keratin bulu ayam broiler sebagai bahan fortifikasi [Microencapsulation of broiler chicken feather keratin extract as a fortification ingredient],” Teknologi Pangan: Media Informasi Dan Komunikasi Ilmiah, vol. 16, no. 1, pp. 106–116, 2025, doi: 10.35891/tp.v16i1.6060.
    40. [40] D. Merlina, “Pengembangan kinerja mikroskop binokular menjadi mikroskop berkamera untuk alat praktikum [Performance development of binocular microscope into camera microscope for practicum equipment],” Indonesian Journal of Laboratory, vol. 4, no. 1, pp. 15–20, 2021, doi: 10.22146/ijl.v4i1.64729.
    41. [41] Y. B. Alam, D. Putri, Y. Fatmadi, N. Faiza, E. Agustina, and R. Purnamasari, “Analysis of differences in functional groups in green tea kombucha using FTIR,” ICAFOHN, vol. 2, no. 1, pp. 113–119, 2024, doi: 10.29080/ichafohn.v2i1.2035.
    42. [42] I. Nugroho, N. Putri, J. Adji, S. Nur, and N. Sekarningrum, “Tinjauan kritis kemampuan fourier transform infrared spectroscopy (FTIR) dalam analisis senyawa obat [A critical review of fourier transform infrared spectroscopy (FTIR) capabilities in drug compound analysis],” Jurnal Ilmiah Wahana Pendidikan, vol. 10, no. 15, pp. 332–344, 2024, doi: 10.5281/zenodo.13777154.
    43. [43] E. K. Dewi and T. S. Nindya, “Hubungan tingkat kecukupan zat besi dan seng dengan kejadian stunting pada balita 6-23 bulan [Relationship between iron and zinc adequacy levels and stunting incidence in toddlers aged 6-23 months],” Amerta Nutrition, vol. 1, no. 4, pp. 361–368, 2017, doi: 10.20473/amnt.v1i4.2017.361-368.
    44. [44] Ministry of Health of the Republic of Indonesia, Regulation of the Minister of Health of the Republic of Indonesia Number 28 of 2019 concerning Recommended Dietary Allowances for the Indonesian Population, Jakarta: Kemenkes RI, 2019.
    45. [45] A. Wulandari, T. C. Sunarti, F. Fahma, and E. Noor, “Karakteristik mikrokapsul antosianin ubi jalar ungu dengan teknik spray drying [Characteristics of purple sweet potato anthocyanin microcapsules using spray drying technique],” Jurnal Teknologi Industri Pertanian, vol. 29, no. 1, pp. 34–44, 2019, doi: 10.24961/j.tek.ind.pert.2019.29.1.34.
    46. [46] C. Hunafa et al., “Imobilisasi mikrokapsul antibakteri minyak asiri eucalyptus globulus pada kain kapas [Immobilization of antibacterial microcapsules of eucalyptus globulus essential oil on cotton fabric],” Arena Tekstil, vol. 36, no. 2, pp. 89–90, 2021, doi: 10.31266/at.v36i2.7345.
    47. [47] National Agency of Drug and Food Control (BPOM RI), Regulation of the Indonesian Food and Drug Authority Number 13 of 2019 concerning Maximum Limits of Microbial Contamination in Processed Food, Jakarta: BPOM RI, 2019.
    48. [48] F. Loe, M. Nugroho, E. Tangkonda, M. Gelolodo, and M. Sanam, “Isolasi dan identifikasi bakteri Salmonella sp. pada ayam broiler [Isolation and identification of Salmonella sp. bacteria in broiler chickens],” Jurnal Veteriner dan Biomedis, vol. 3, no. 1, pp. 9–15, 2025, doi: 10.29244/jvetbiomed.3.1.9-15.
    49. [49] T. S. Palus, M. U. E. Sanam, and A. I. R. Detha, “Identification Of Salmonella Sp and Escherichia coli in flies captured at meat selling area in naikoten traditional market, Kupang City," Jurnal Veteriner Nusantara, vol. 1, no. 1, pp. 10–13, 2016, doi: 10.35508/jvn.v9i2.
    50. [50] Y. Rasyadi, F. Rahim, and N. Handayani, “Formulasi krim dari mikrokapsul papain [Formulation of cream from papain microcapsules],” JAFP (Jurnal Akademi Farmasi Prayoga), vol. 5, no. 1, pp. 32–39, 2024, doi: 10.56350/jafp.v5i1.73.

Categories